Pseudomonas aeruginosa is a kind of representative indigenous bacterium during environment.
In the hospital-acquired infections, the Multiple-Drug-Resistant Pseudomonas aeruginosa (MDRP) which got tolerance for a main antibiotic widely become the problem.
The PCR-based ORF Typing (POT) developed by Dr.Suzuki of Aichi Prefectural Institute of Public Health and Dr.Iinuma of Kanazawa Medical University is a method to amplify plural specific genes by Multiplex PCR at the same time, and then to analyze agarose gel electrophoresis banding patterns.
Features
- Required time is approximately 4 hours.
- Nearly the same discriminability as the Pulsed Field Gel Electrophoresis(PFGE) and Multilocus Sequence Typing (MLST).
- This POT KIT can compare the homogeny between strains by digitizing (POT type) and analysis result.
Composition
Reagent | Volume |
---|---|
A AptaTaq DNA Master(5×Conc.) | 500 μL×1 |
B PCR supplement | 500 μL×1 |
C Primer mix α | 250 μL×1 |
D Primer mix β | 250 μL×1 |
E Positive control | 500 μL×1 |
F Loading buffer (6×Conc.) | 500 μL×1 |
Product Information
Product Code | Product Name | Pack Size | Stored at |
---|---|---|---|
08187-96 | CicaGeneusTM Pseudo POT KIT | 50 rxns | -20 ℃ ~ -25 ℃ |
08178-96 | CicaGeneusTM DNA Extraction Reagent | 120 rxns | 2 ℃~8 ℃ |
Multiplex PCR kit series (stored at -20℃ ~ -25 ℃)
Product No. | Product Name | Package size |
---|---|---|
08180-96 | Cica GeneusTM Staph POT KIT | 120 rxns |
08187-96 | Cica GeneusTM Pseudo POT KIT | 50 rxns |
08062-96 | Cica GeneusTM Acineto POT KIT | 30 rxns |
08087-96 | Cica GeneusTM Pathogenesis Gene Detection PCR Kit(for DEC) | 100 rxns |
08179-96 | Cica GeneusTM Staph coaglase detection set | 50 rxns |
08081-96 | Cica GeneusTM Bovine Mycoplasma HI screening kit | 100 rxns |
Related Reagents
Product Code | Product Name | Packaging |
---|---|---|
46510-79 | 10 × TBE Buffer | 1 L |
46509-79 | 10 × TAE Buffer | 1 L |
14575-43 | Ethidium bromide solution | 10 mL |
01089-23 | Agarose KANTO HC | 100 g |
01016-96 | AptaTaq DNA Master(5×Conc.) | 500 μL |