Collagen-Based Cell Culture Insert for Advanced Co-Culture & Permeability Assays

ad-MED Vitrigel™ 2 is a next-generation cell culture insert that utilizes a Vitrigel™ membrane—a high-density, collagen-only membrane produced by a unique vitrification process. Unlike conventional synthetic membranes (PET/PTFE), the Vitrigel™ membrane provides enhanced transparency, permeability, and biocompatibility, making it ideal for advanced in vitro tissue modeling and drug absorption studies.

Vitrigel™ is a registered trademark of NARO (National Agriculture and Food Research Organization), Japan.

  • Biomimetic Collagen Membrane
    Composed entirely of bovine collagen—ideal for constructing physiologically relevant tissue models.
  • Superior Transparency & Imaging Quality
    High clarity even when hydrated—enables excellent observation under microscopy.
    Low autofluorescence for high-contrast fluorescent imaging.
  • Excellent Permeability
    Higher permeability than PET (0.4 μm) membranes.
    Molecular weight-dependent transport using FITC-dextran (FD4, FD10, FD40).
  • Innovative Co-Culture Capability
    Allows simultaneous cell seeding on both sides of the membrane using optional ring inserts.
  • Robust Membrane Handling
    Channel-like structure supports easier pipetting and prevents cell detachment during media changes.

Technical Comparison

FeaturePET Membrane (0.4 µm)PTFE MembraneVitrigel™ Collagen Membrane
TransparencyModerateLow✅ High
AutofluorescenceModerateHigh✅ Low
Permeability (FD4–FD40)LowLow✅ High & MW-dependent
Cell CompatibilityStandardVariable✅ Biomimetic Collagen
Product Lineup
PRODUCT CODE ITEM GRADE SIZE
08363-96 ad-MED Vitrigel™ 2 (12 well) for cell culture 12 pieces/set
08364-96 ad-MED Vitrigel™ 2 (24 well) for cell culture 24 pieces/set
08368-96 ad-MED Vitrigel™ 2 (96 well) for cell culture 96 well/set
44125-67 Vitrigel™ 2 Membrane (φ15 ㎜), sterlie for cell culture 1 pack (24 sheets)
44125-67 Vitrigel™ 2 Membrane (φ21 ㎜), sterlie for cell culture 1 pack (24 sheets)
08369-96 Option Ring for ad-MED Vitrigel™ (12 well) for cell culture 1 pack (24 pieces)
08373-96 Option Ring for ad-MED Vitrigel™ (24 well) for cell culture 1 pack (24 pieces)
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