Fast Multiplex PCR Detection of Plasmid-Mediated AmpC β-Lactamase Genes

The Cica Geneus AmpC Genotype Detection KIT enables rapid and accurate identification of six major plasmid-mediated AmpC β-lactamase genes, which are often responsible for resistance to cephalosporins and cephamycins. This multiplex PCR kit is ideal for infection control, antimicrobial resistance (AMR) surveillance, and epidemiological studies of resistant Gram-negative bacteria.

Key Features

  • Detects 6 AmpC Gene Families
    Targets the most clinically relevant plasmid-mediated genes:
  • CIT family
  • FOX family
  • ACT family
  • DHA family
  • ACC family
  • MOX family
  • Two-Tube Multiplex PCR System
    Designed for high specificity using two primer sets for accurate identification.
  • Fast & Efficient
    Total assay time is approximately 3 hours using a standard thermal cycler.
  • Electrophoresis-Based Interpretation
    Amplicon sizes are distinct and easily visualized with agarose gel electrophoresis.
  • Validated by Experts
    Co-developed with Hiroshima University.
  • Research Use
    Supports research into ESBL/AmpC resistance patterns, surveillance, and infection control protocols.
PRODUCT LIST
PRODUCT CODE ITEM PACKAGE SIZE STORAGE
08143-96 Cica Geneus AmpC Genotype Detection KIT 30 rxns –20°C to –25°C
08178-96 Cica Geneus™ DNA Extraction Reagent 120 rxns 2°C to 8°C

MULTIPREX PCR kit series

PRODUCT CODE ITEM PACKAGE SIZE STORAGE
08180-96 Cica Geneus Staph POT Kit 120 rxns -25℃~-20℃
08180-97 30 rxns
08187-96 Cica Geneus Pseudo POT KIT 50 rxns -25℃~-20℃
08062-96 Cica Geneus Acineto POT KIT 30 rxns -25℃~-20℃
08362-97 Cica Geneus E. coli POT KIT 30 rxns -25℃~-20℃
08106-97 Cica Geneus C.diff POT KIT 30 rxns -25℃~-20℃
08143-96 Cica Geneus AmpC Genotype Detection KIT 30 rxns -20 ℃~-25 ℃
08116-96 Cica Geneus ESBL Genotype Detection Kit 30 rxns -25℃~-20℃
08158-96 Cica Geneus Carbapenemase Genotype Detection KIT2 30 rxns -25℃~-20℃
08187-96 Cica Geneus Pathogenesis Gene Detection PCR Kit 30 rxns -25℃~-20℃

Related Reagent

PRODUCT CODE ITEM PACKAGE SIZE
46510-79 10×TBE buffer 1 L
46509-79 10×TAE buffer 1 L
14575-43 Ethidium bromide solution 10 mL
01089-23 Agarose KANTO HC 100 g
01098-23 Agarose KANTO LE 100 g
01016-96 AptaTaq DNA Master (5×Conc.) 500 μL
⚠ Important Notes
  • ⦿ Some rare AmpC subtypes may not be detectable by this kit.
  • ⦿ Chromosomal AmpC genes in certain bacterial species may also yield positive results.
  • ⦿ This kit does not identify bacterial species.
  • ⦿ Resistance may vary based on gene expression or mutation, even if the gene is detected.