For Detection of Diarrheagenic Escherichia coli (DEC) Pathotypes

The Cica Geneus Pathogenesis Gene Detection PCR Kit is a multiplex PCR-based diagnostic solution for detecting major virulence genes in diarrheagenic E. coli. This kit enables the identification of E. coli pathotypes responsible for gastrointestinal disease, such as EHEC, EPEC, ETEC, EIEC, and EAEC. By amplifying multiple genes simultaneously and digitizing results, laboratories can swiftly interpret electrophoresis band patterns and classify isolates with confidence.

Key Features

  • Multiplex Detection of 5 DEC Pathotypes
    Targets key virulence genes associated with:
  • EHEC (Enterohemorrhagic)
  • EPEC (Enteropathogenic)
  • ETEC (Enterotoxigenic)
  • EIEC (Enteroinvasive)
  • EAEC (Enteroaggregative)
  • Amplifies 8 Genetic Targets
    Includes eaeA, stx1, stx2, est, elt, aggR, invE, and internal control (16S rDNA).
  • Clear & Objective Results
    Band presence is analyzed with a point-based system to determine total virulence and pathotype classification.
  • Rapid & Efficient
    Easy to implement using conventional PCR and agarose gel electrophoresis. Interpretation takes just minutes post-run.
  • Ready-to-use Reagents
    Includes all essential primer mixes and controls for 100 reactions.

PRODUCT LIST

PRODUCT CODE ITEM PACKAGE SIZE STORAGE
08187-96 Cica Geneus™ Pathogenesis Gene Detection PCR Kit 100 rxns –20°C to –25°C
08178-96 Cica Geneus™ DNA Extraction Reagent 120 rxns 2°C to 8°C

MULTIPREX PCR kit series

PRODUCT CODE ITEM PACKAGE SIZE STORAGE
08180-96 Cica Geneus Staph POT Kit 120 rxns -25℃~-20℃
08180-97 30 rxns
08187-96 Cica Geneus Pseudo POT KIT 50 rxns -25℃~-20℃
08062-96 Cica Geneus Acineto POT KIT 30 rxns -25℃~-20℃
08362-97 Cica Geneus E. coli POT KIT 30 rxns -25℃~-20℃
08106-97 Cica Geneus C.diff POT KIT 30 rxns -25℃~-20℃
08143-96 Cica Geneus AmpC Genotype Detection KIT 30 rxns -20 ℃~-25 ℃
08116-96 Cica Geneus ESBL Genotype Detection Kit 30 rxns -25℃~-20℃
08158-96 Cica Geneus Carbapenemase Genotype Detection KIT2 30 rxns -25℃~-20℃
08187-96 Cica Geneus Pathogenesis Gene Detection PCR Kit 30 rxns -25℃~-20℃

Related Reagent

PRODUCT CODE ITEM PACKAGE SIZE
46510-79 10×TBE buffer 1 L
46509-79 10×TAE buffer 1 L
14575-43 Ethidium bromide solution 10 mL
01089-23 Agarose KANTO HC 100 g
01098-23 Agarose KANTO LE 100 g
01016-96 AptaTaq DNA Master (5×Conc.) 500 μL